Principal Investigator
email: ayalashiber@technion.ac.il
Phone: +972-778873763
Address: Emerson, room 3-26
Our main research objective is to resolve the long-standing question: How do cells direct their proteome to fold to their native, functional state and avoid misfolding diseases. We are explorִִִing the mechanisms guiding the folding and assembly of newly synthesized proteins into multi-molecular complexes as well as the mechanisms for degradation of “lonely” subunits. We are studying the role of the ribosome as a platform for coordinating complex assembly during synthesis, by advanced techniques combining biochemistry and deep sequencing such as selective ribosome profiling as well as super-resolution microscopy. We are also developing tools for studying single-molecule mRNA-protein interactions, in vivo.
Lab Manager & Research associate
Hagit Bar-Yosef, Ph.D
email: hagitby@technion.ac.il
Phone: +972-778873763
Address: Emerson, room 3-24
BSc in molecular biology at the Department of Life Sciences, Bar-Ilan University, Israel. Master’s degree at Shimon Pollack`s lab, at the Department of Immunology in the Faculty of Medicine at the Technion-Israel Institute of Technology “Flagellin activation of Toll-Like Receptor 5 directs human monocytes into distinct differentiation towards dendritic cells”. Phd in Daniel Kornitze`s lab at the Department of Molecular Microbiology in the Faculty of Medicine at the Technion “Genetic and pharmacological suppression of fungalmorphogenesis”. In her work on Candida albicans, she showed that endocytosis is both essential and highly regulated in hyphal morphogenesis. Since 2017 she is working at the Biology Faculty, Technion. She first worked as head of student labs for bachelor’s studies at the faculty. In October 2019 she joined the lab and is working on co-translational protein folding.
Students
Johannes A. Venezian
Ph.D student
B.A. in Physics and Mathematics, Technion, Israel. Graduation project, under prof. Enrique Kajomovitz’s supervision, Toy Monte Carlo simulation for the production of jets and their reconstruction in the ROOT environment.
Current Research: Evolution of protein folding and assembly pathways: Deciphering the characteristics of divergent co-translational assembly pathways.
Junyi He
Ph.D student
B.Sc: College of Plant Protection, Northwest A&F University, China. Thesis: “GST pulldown assay between the wheat stripe rust effector protein RTP1 and its candidate target protein, Nudix23”. M.Sc: The Jacob Blaustein Institute for Desert Research, Ben Gurion University of the Negev, Simon Barak`s lab. Thesis: “Molecular mechanisms of long and short term salt mediated inhibition of seed germination of Eutrema salsugineum and Schrenkiella parvula”.
Current Research: Discovery of assembly-specific degradation factors: A systematic, proteome-wide identification of novel, co-translationally acting degradation factors.
Qamar Shhade
M.Sc. student
B.Sc. in Medical Sciences and Biology, University of Haifa, Israel. Thesis: “Cytosolic dsDNA emergence following macrophage efferocytosis might lead to STING mediated production of IFN beta during the resolution of inflammation”.
Current Research: mRNA spatial organization in the cell.
Muhammad Makhzumy
M.Sc. student
email: muhammad@mmcmed.co
B.Sc in Biotechnology and food engineering, Technion, Israel. Master of Engineering, Biotechnology and food engineering, Technion, Israel. Protein Purification team lead ,Stem Cells and Bioreactors logistics manager, Vaccines production manager and Medical Device Quality Assurance manager.
Current research: single molecule approaches to ribosome profiling.
Hila Zilberman
M.Sc. student
B.Sc in Biotechnology and food engineering, Technion, Israel.
Current research: Mechanistic analysis of ISGylation at the ribosome following viral infection.
Project Students
Daniel Dahan
Project student
Data Science & Engineering student.
Project: Computational modeling of co-translational interface folding
Alumni
Shoham Alhanati
Project student
Structural characterization and Modelling of co-translationally assembling Protein Complexes
Maayan Cohen
Project student
Prediction of NatB complex structurein Saccharomyces cerevisiae
Tamar Gilinski
Project student
Prediction of NatB complex structurein Saccharomyces cerevisiae
Seraj Attarya
M.Sc. student
B.Sc. in Medical Sciences and Biology, Technion, Israel. Thesis: “Myelodysplastic syndrome link to Acute Myeloid Leukemia”.
Research: Discovery of assembly-specific degradation factors: A systematic, proteome-wide identification of novel, co-translationally acting degradation factors.